Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.095277 |
Chromosome: | chromosome 5 |
Location: | 3398013 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre05.g241150 | (1 of 1) K11491 - condensin-2 complex subunit D3 (NCAPD3) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGAAGGGGGGGGGTGTAGATACCAGCCC |
Internal bar code: | CAGCAGCCCCTGTAAGGCCTCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 459 |
LEAP-Seq percent confirming: | 90.7201 |
LEAP-Seq n confirming: | 2444 |
LEAP-Seq n nonconfirming: | 250 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AACTAAGGGGGACAGTGGCT |
Suggested primer 2: | CGGTAGACTGGTGTGGTGTG |