Insertion junction: LMJ.RY0402.095284_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.095284
Chromosome:chromosome 3
Location:576442
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g145487 (1 of 5) IPR001841//IPR013083 - Zinc finger, RING-type // Zinc finger, RING/FYVE/PHD-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCATGCGCGCGCGCCGCGAGGCCGCCAAC
Internal bar code:CGGCTCACGACGTGGGGGGAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:542
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:51
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTCACCTTGACTCGCTCCTC
Suggested primer 2:ACCTTCGGTCTCAGTCGTGT