Insertion junction: LMJ.RY0402.095284_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.095284
Chromosome:chromosome 3
Location:576442
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g145487 (1 of 5) IPR001841//IPR013083 - Zinc finger, RING-type // Zinc finger, RING/FYVE/PHD-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCTGGTCCTTGTCTCCCAGGTCACTGCG
Internal bar code:GAGTTCGATTCTTGTCCATTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:347
LEAP-Seq percent confirming:99.6081
LEAP-Seq n confirming:9404
LEAP-Seq n nonconfirming:37
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTTCGGTCTCAGTCGTGT
Suggested primer 2:GTCACCTTGACTCGCTCCTC