| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.095300 |
| Chromosome: | chromosome 12 |
| Location: | 5265312 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528500 | MTH1,NUDT1 | (1 of 1) 3.6.1.56 - 2-hydroxy-dATP diphosphatase / Oxidized purine nucleoside triphosphatase; Oxidized purine nucleoside triphosphatase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCTCCTTACCCAGCCCCACAAGGCTGG |
| Internal bar code: | GGTTACAGGGAGATTTGCAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 316 |
| LEAP-Seq percent confirming: | 99.417 |
| LEAP-Seq n confirming: | 2217 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGATGTAAACGATGCCAC |
| Suggested primer 2: | GACAAGGTGACGGACAGGAT |