| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.095313 |
| Chromosome: | chromosome 10 |
| Location: | 6475967 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g466300 | AAAS1 | (1 of 1) PTHR14494//PTHR14494:SF0 - ALADIN/ADRACALIN/AAAS // ALADIN; Aladin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAGCCAGCCGCTCGCCCGCCGCATCCCA |
| Internal bar code: | GTTTAGGTCTTAATGTCCGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 32 |
| LEAP-Seq percent confirming: | 99.5796 |
| LEAP-Seq n confirming: | 4501 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGACACTGCCACTGCTG |
| Suggested primer 2: | GCTCACTTCACACTGCCTCA |