| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.095324 |
| Chromosome: | chromosome 16 |
| Location: | 1761958 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654992 | (1 of 1) 2.1.1.225 - tRNA:m(4)X modification enzyme | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTTTGCTTCTGCTGCCGCTGCTTCTGC |
| Internal bar code: | TTCGTGCATATGTAGACGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 351 |
| LEAP-Seq percent confirming: | 54.2553 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGACCTCCTGTACGCAA |
| Suggested primer 2: | TTGTTGTTGGTGGTGTCAGG |