Insertion junction: LMJ.RY0402.095385_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.095385
Chromosome:chromosome 9
Location:6946547
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g410500 PAP11 (1 of 1) IPR002934//IPR023214 - Polymerase, nucleotidyl transferase domain // HAD-like domain; Class-II RNA nucleotidyl transferase 11 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAACTCCTCATCATCCATGCCCTCCCCA
Internal bar code:ACGTTACTCAGATTGCAAGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:497
LEAP-Seq percent confirming:99.5825
LEAP-Seq n confirming:1908
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CACAATACACGCACAGGTCC
Suggested primer 2:GAAGGTACGACAGCTGAGCC