| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.095398 |
| Chromosome: | chromosome 12 |
| Location: | 1024236 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g491300 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCTGAGGAAACTCGCAGGAAACTCGGCT |
| Internal bar code: | CGGGGTGTGTATCGATAAGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 625 |
| LEAP-Seq percent confirming: | 97.0115 |
| LEAP-Seq n confirming: | 422 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGCACCAAGACACACAT |
| Suggested primer 2: | CTTCCCCGCTTACATGTGTT |