| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.095429 |
| Chromosome: | chromosome 6 |
| Location: | 3597068 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278123 | VPS39 | (1 of 781) IPR000104 - Antifreeze protein, type I; Subunit of VPS-C complex | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTCTCTATACCCCTGACTGCAATACGGT |
| Internal bar code: | GCATCTCTCAAGCTGGTTCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 152 |
| LEAP-Seq percent confirming: | 93.1362 |
| LEAP-Seq n confirming: | 882 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTCCCCGTAACTGCATTG |
| Suggested primer 2: | GGAGACAAGGCACATGGTTT |