| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.095437 |
| Chromosome: | chromosome 7 |
| Location: | 1481306 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g323650 | (1 of 1) PTHR13109//PTHR13109:SF7 - NEUROCHONDRIN // NEUROCHONDRIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCTTGCCCCCACTCTTGCACAAACCTG |
| Internal bar code: | GCCAGACGACGTGCTCCCCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 69 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCACGGTAAACAGTCGCT |
| Suggested primer 2: | CTCATCTCCCACTACCGCTC |