Insertion junction: LMJ.RY0402.095440_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.095440
Chromosome:chromosome 2
Location:1693195
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g085900 IPP1,IMP1 Inositol-phosphate phosphatase; (1 of 2) K01092 - myo-inositol-1(or 4)-monophosphatase (E3.1.3.25, IMPA, suhB) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACTTGGACCACGCGCACCTTCACAGCGGC
Internal bar code:AACACTGACGTAAGCTCGCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:645
LEAP-Seq percent confirming:99.4637
LEAP-Seq n confirming:2040
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGTGACTGAGACGGACAA
Suggested primer 2:GTCCCATGGCTTCAAGTTGT