Insertion junction: LMJ.RY0402.095450_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.095450
Chromosome:chromosome 1
Location:7184984
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCACCCGCGGGTGCGACCACCAAGCCACA
Internal bar code:GTGCCAATCGCATAACGGGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:200
LEAP-Seq percent confirming:99.4966
LEAP-Seq n confirming:593
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AACATTGGCGTTTCTTGGTC
Suggested primer 2:CATCCACCCTCTACCCTTCA