| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.095453 |
| Chromosome: | chromosome 15 |
| Location: | 1456972 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g643028 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCGCTGGTAGGCCTCGTGCGTCTCCTCG |
| Internal bar code: | CCAAAATACATAGCGACCGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 382 |
| LEAP-Seq percent confirming: | 97.8383 |
| LEAP-Seq n confirming: | 5929 |
| LEAP-Seq n nonconfirming: | 131 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTCTCGCAAACAGACAC |
| Suggested primer 2: | AAGTGGCGGATCTAGAAGCA |