| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.095475 |
| Chromosome: | chromosome 17 |
| Location: | 2095534 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g712250 | (1 of 4) 2.7.11.13 - Protein kinase C / PKC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGATGCGAAACACATCCTTAGCCTGTCAT |
| Internal bar code: | ATTGTGATTTCTTCATATACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 66 |
| LEAP-Seq percent confirming: | 99.7886 |
| LEAP-Seq n confirming: | 472 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGATGCTCCTGGACTTTGG |
| Suggested primer 2: | TGTAGGCTCAGAGGGAAACG |