| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.095528 |
| Chromosome: | chromosome 6 |
| Location: | 2040932 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g264450 | AOT5 | Amino acid transporter; (1 of 1) K14994 - solute carrier family 38 (sodium-coupled neutral amino acid transporter), member 7/8 (SLC38A7_8) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATGCGGGACCGCTAGCGCTGCGTGCCG |
| Internal bar code: | CCCACATAGACGCCTGTGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 505 |
| LEAP-Seq percent confirming: | 98.9967 |
| LEAP-Seq n confirming: | 888 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGTTTAGTTTGGGCTGG |
| Suggested primer 2: | TAGCTGGCTGTTCACATTGG |