| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.095546 |
| Chromosome: | chromosome 7 |
| Location: | 301925 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314250 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCCAGCAATGTGCCAGCTCCCGTGCGGT |
| Internal bar code: | GTTCCGGTGCGAGCCTCGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 600 |
| LEAP-Seq percent confirming: | 98.9734 |
| LEAP-Seq n confirming: | 6074 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGATAGTTGGTGTGGGAGC |
| Suggested primer 2: | TAGCGAGAGGGTGACAGAGG |