| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.095577 |
| Chromosome: | chromosome 2 |
| Location: | 137195 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g074250 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCTGTCGGGTCTGCCCGCCAGCACCGC |
| Internal bar code: | TACCACAGCGTCGATAATACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 337 |
| LEAP-Seq percent confirming: | 17.8082 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGACGATGAGGAGGAGG |
| Suggested primer 2: | TGAGTGACCACGACGAGAAG |