| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.095581 |
| Chromosome: | chromosome 6 |
| Location: | 5401293 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g283900 | (1 of 1) PTHR16007//PTHR16007:SF15 - EPIDIDYMAL MEMBRANE PROTEIN E9-RELATED // TRANSMEMBRANE PROTEIN 45B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGAAGAGTGGGAGGCAGAGAGGAGGAAG |
| Internal bar code: | GTGGTGCAAGTGTCGACGAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 774 |
| LEAP-Seq percent confirming: | 98.8659 |
| LEAP-Seq n confirming: | 4446 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATAAGGGGAGGGGTGTTTT |
| Suggested primer 2: | TGCGTGTTCTTACTTGTCGC |