| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.095604 |
| Chromosome: | chromosome 3 |
| Location: | 6119073 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g191776 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTGCGTGAATGCAGGTTCAGCATTGAG |
| Internal bar code: | AGATTGAGACTCGGTACGTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 101 |
| LEAP-Seq percent confirming: | 98.5636 |
| LEAP-Seq n confirming: | 4323 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTGTCCGAACGAATACGG |
| Suggested primer 2: | GACTGCTCACCCCGTTACAT |