Insertion junction: LMJ.RY0402.095624_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.095624
Chromosome:chromosome 11
Location:1166039
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467688 (1 of 1) 1.2.3.7 - Indole-3-acetaldehyde oxidase / Indoleacetaldehyde oxidase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGATTGGGCTCGCCAGCGGAAGCGTTTA
Internal bar code:TGGTTCGGACATGTAATTCACG

Confirmation - LEAP-Seq

LEAP-Seq distance:982
LEAP-Seq percent confirming:97.4227
LEAP-Seq n confirming:189
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CACACGTACCCTCCGAAGAT
Suggested primer 2:AGGCTAGCGAGTGGAAAACA