| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.095657 |
| Chromosome: | chromosome 16 |
| Location: | 4775124 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686550 | (1 of 1) PF12400 - Vaculolar membrane protein (DUF3661) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTGTGCATACGGTAGGCAGGTAGAAAAC |
| Internal bar code: | CATGTCGTTCTTGTGCGACTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 849 |
| LEAP-Seq percent confirming: | 98.7611 |
| LEAP-Seq n confirming: | 3906 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGTGATGGACAAGGCTCC |
| Suggested primer 2: | CACACAATGATCACAGCACG |