Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.095658 |
Chromosome: | chromosome 8 |
Location: | 4195356 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre08.g380201 | SHKD1 | (1 of 1) 4.2.1.10 - 3-dehydroquinate dehydratase; Putative dehydroquinate dehydratase/shikimate:NADP oxidoreductase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGGCCAAACCTCCTGCGCTCCCTGTGG |
Internal bar code: | CGTCTTGCACTGGTGGCAGAAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 426 |
LEAP-Seq percent confirming: | 27.6699 |
LEAP-Seq n confirming: | 57 |
LEAP-Seq n nonconfirming: | 149 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGGGTGTAGTGTGCGTGTTT |
Suggested primer 2: | AAGCCTGAAGAACGAGTGGA |