| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.095674 |
| Chromosome: | chromosome 4 |
| Location: | 1829680 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g211750 | (1 of 2) K07297 - adiponectin receptor (ADIPOR) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTAAGGCATGACTAGCATTGCACAGCCT |
| Internal bar code: | GTCTTGGTATCGTGCCGATTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 908 |
| LEAP-Seq percent confirming: | 99.7106 |
| LEAP-Seq n confirming: | 11370 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAGGATGAAGGTGAGGT |
| Suggested primer 2: | AAGGTTGAGTGGACTGGGTG |