| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.095681 |
| Chromosome: | chromosome 10 |
| Location: | 5500632 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g459100 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAATCGTTGCATCTAGGCCTCAGTGCAG |
| Internal bar code: | CGCATTTACAGAGTGGCTAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1064 |
| LEAP-Seq percent confirming: | 98.6426 |
| LEAP-Seq n confirming: | 5959 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACATTACAACACGCAACCG |
| Suggested primer 2: | CCCTTCACCACGCTTACAAT |