| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.095682 |
| Chromosome: | chromosome 6 |
| Location: | 8531575 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g308100 | ECH2 | Enoyl-CoA hydratase 2; (1 of 2) 4.2.1.119 - Enoyl-CoA hydratase 2 / ECH2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCATGAAATATGGTGAACTGAGCGCGTGC |
| Internal bar code: | ATTCTTCTCGCTGCGGACAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 292 |
| LEAP-Seq percent confirming: | 87.5642 |
| LEAP-Seq n confirming: | 3922 |
| LEAP-Seq n nonconfirming: | 557 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTCCTTATCGGGCTTGTT |
| Suggested primer 2: | GACAGTGACCAGTCCAGCCT |