Insertion junction: LMJ.RY0402.095684_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.095684
Chromosome:chromosome 6
Location:3929734
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278186 (1 of 1) IPR000157//IPR011251//IPR016024 - Toll/interleukin-1 receptor homology (TIR) domain // Luciferase-like domain // Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGCGTGTGCGGGCGTGCGTATTTTGTGC
Internal bar code:CCTATATTGCAACTAAGAGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:152
LEAP-Seq percent confirming:99.7642
LEAP-Seq n confirming:846
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CAACCTTGATCCCTCCTTGA
Suggested primer 2:AACCACAACAAAACGAAGCC