| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.095684 |
| Chromosome: | chromosome_10 |
| Location: | 4256651 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g450450 | FAP18 | Flagellar Associated Protein, serine endopeptidase | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGAGCGGCGGCGCTCGTCCTGCGACGGCG |
| Internal bar code: | CTCGTGTTACTGCCGACGGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 128 |
| LEAP-Seq percent confirming: | 47.1681 |
| LEAP-Seq n confirming: | 508 |
| LEAP-Seq n nonconfirming: | 569 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCTCCGTGTACTTGAGC |
| Suggested primer 2: | GGGTAGCACATCGGTCAAGT |