Insertion junction: LMJ.RY0402.095684_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.095684
Chromosome:chromosome 10
Location:4256651
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre10.g450450 FAP18 Flagellar Associated Protein 18; (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAGCGGCGGCGCTCGTCCTGCGACGGCG
Internal bar code:CTCGTGTTACTGCCGACGGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:128
LEAP-Seq percent confirming:47.1681
LEAP-Seq n confirming:508
LEAP-Seq n nonconfirming:569
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCCTCCGTGTACTTGAGC
Suggested primer 2:GGGTAGCACATCGGTCAAGT