| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.095688 |
| Chromosome: | chromosome 12 |
| Location: | 8880248 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544650 | (1 of 10) PF13489 - Methyltransferase domain (Methyltransf_23) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACGCCCGCATACGCCGGCCGCAGCTCGG |
| Internal bar code: | GCGCCCCTTACAATTGAACAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 163 |
| LEAP-Seq percent confirming: | 95.38 |
| LEAP-Seq n confirming: | 640 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCCACGGTAGTTAGCAGA |
| Suggested primer 2: | ATAAGCAGTCGAAGGCAGGA |