| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.095713 |
| Chromosome: | chromosome 16 |
| Location: | 5607208 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679850 | CYG35 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTCCCCCGCCATAACAGTGGAGCGCGGC |
| Internal bar code: | ACGTTGTCCACGTCAGCGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 170 |
| LEAP-Seq percent confirming: | 72.0963 |
| LEAP-Seq n confirming: | 509 |
| LEAP-Seq n nonconfirming: | 197 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCCACTCTCTCACCTGG |
| Suggested primer 2: | ATCTCAGGTGTCTGGGCATC |