| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.095729 |
| Chromosome: | chromosome_8 |
| Location: | 3411876 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g374650 | p44,TPR5,DII3 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGTTCCACACGACGCCTCCAGCTGCCGCTA |
| Internal bar code: | ATACGTGCATGCATGCTACGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 523 |
| LEAP-Seq percent confirming: | 96.6897 |
| LEAP-Seq n confirming: | 701 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGTTTAGTTTGGGCTGG |
| Suggested primer 2: | GAGCAACAGAAACTGCCACA |