Insertion junction: LMJ.RY0402.095755_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.095755
Chromosome:chromosome 3
Location:1632192
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g152900 MPA5 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 20) 3.1.4.17 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGGAGGTAGGTGCCGTGTGTGTTGGGCG
Internal bar code:TAATTTTTGTCTTGGTGTGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:981
LEAP-Seq percent confirming:98.6186
LEAP-Seq n confirming:1642
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTACGTCCCGTACGTGTGTG
Suggested primer 2:CAGTTCGCCAAGTCAGTGAA