| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.095763 |
| Chromosome: | chromosome 16 |
| Location: | 7109851 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680117 | (1 of 1) K15692 - E3 ubiquitin-protein ligase RNF13 [EC:6.3.2.19] (RNF13, RZF) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCGTACACAATGGCAGCAAGCGCGCCCG |
| Internal bar code: | CAGGAGCGAGATTACGATTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 94 |
| LEAP-Seq percent confirming: | 87.0057 |
| LEAP-Seq n confirming: | 154 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCGCATCTGCGATATCTA |
| Suggested primer 2: | CTTCTGTGCGACAGGAATGA |