| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.095768 |
| Chromosome: | chromosome 9 |
| Location: | 647476 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g403300 | (1 of 1) K03144 - transcription initiation factor TFIIH subunit 4 (TFIIH4, GTF2H4, TFB2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAAGGTTTCCGCACGAGCGTAGCCGGTA |
| Internal bar code: | GTAGCGTGGTAAAATACATGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 234 |
| LEAP-Seq percent confirming: | 99.2248 |
| LEAP-Seq n confirming: | 128 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATGGTCCATCACCTCTA |
| Suggested primer 2: | CTTCCTCTCCTCCTCCTCGT |