| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.095836 |
| Chromosome: | chromosome 10 |
| Location: | 6326506 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465300 | (1 of 89) 3.6.4.13 - RNA helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCGGCAGCCGGTGCCGACACCAGTGGT |
| Internal bar code: | TGTCACGCTATTGTAGTATATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2 |
| LEAP-Seq percent confirming: | 99.6193 |
| LEAP-Seq n confirming: | 3925 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGTGAAGGCGTAGCCAG |
| Suggested primer 2: | CTGTACTTCACCGCGCCT |