| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.095856 |
| Chromosome: | chromosome 1 |
| Location: | 5859500 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041855 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTGTCCCCCTCACGTCTGCCTCTCCCT |
| Internal bar code: | CTTCTCTGGTTGGCAGCGCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 9 |
| LEAP-Seq percent confirming: | 97.2789 |
| LEAP-Seq n confirming: | 286 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCTCTTCAGTCTCCACC |
| Suggested primer 2: | AGTGTGGTGGGGGTATGTGT |