| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.095861 |
| Chromosome: | chromosome 1 |
| Location: | 5025710 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034700 | (1 of 3) PF12843 - Putative quorum-sensing-regulated virulence factor (QSregVF_b) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGTCGCCGCCCTCAGACGCACATCTGGA |
| Internal bar code: | ATAGTAGGGAGCTGTGGTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 305 |
| LEAP-Seq percent confirming: | 83.1822 |
| LEAP-Seq n confirming: | 1009 |
| LEAP-Seq n nonconfirming: | 204 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCGCGTGTATTTGGTGTT |
| Suggested primer 2: | CGGGCACGCATTTCTATAAT |