| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.095878 |
| Chromosome: | chromosome 3 |
| Location: | 3845661 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170700 | (1 of 3) 3.2.1.39 - Glucan endo-1,3-beta-D-glucosidase / Laminarinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGTGCTAGCTTGCCACACCCTGCCCGTT |
| Internal bar code: | GGTGGTGGTCGCTCATTGCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 208 |
| LEAP-Seq percent confirming: | 83.248 |
| LEAP-Seq n confirming: | 4229 |
| LEAP-Seq n nonconfirming: | 851 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCGATATGCTTGCTGGTG |
| Suggested primer 2: | CAGTCAATGGAGGGCTTTGT |