| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.095890 |
| Chromosome: | chromosome 16 |
| Location: | 1150445 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650350 | (1 of 1) PF03457 - Helicase associated domain (HA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGATCTTATACGGCTTGTCAATCTCTAC |
| Internal bar code: | TCCAGTGCGAGTCTGATCCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 234 |
| LEAP-Seq percent confirming: | 99.6619 |
| LEAP-Seq n confirming: | 1179 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCAGGGACTGACCAGTGT |
| Suggested primer 2: | CGCTCTCTATGCTTCCATCC |