| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.095896 |
| Chromosome: | chromosome 1 |
| Location: | 4442191 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g030150 | (1 of 1) PTHR13489:SF0 - MINI-CHROMOSOME MAINTENANCE COMPLEX-BINDING PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCTTGTAAATGTCTACATGCTGAACTGA |
| Internal bar code: | TAAATCCGTACGTCGCCTTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 141 |
| LEAP-Seq percent confirming: | 33.3969 |
| LEAP-Seq n confirming: | 175 |
| LEAP-Seq n nonconfirming: | 349 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGTTCCTAGCCTCTCGG |
| Suggested primer 2: | GACTGTACGAGAGCATGCCA |