Insertion junction: LMJ.RY0402.095924_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.095924
Chromosome:chromosome_12
Location:1217226
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre12.g489500 antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GAAAAGAACTGAACGAGTGGAGTCACGAAT
Internal bar code:GCGAGTTGGCGCGTGATCTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1170
LEAP-Seq percent confirming:99.4552
LEAP-Seq n confirming:3651
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TGACCTGACTGGAGCAGTTG
Suggested primer 2:CCGCTATACGAACGGAACAT