| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.095968 |
| Chromosome: | chromosome 3 |
| Location: | 1706758 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTTAACCCAGACCAGCCCGTCTGTTGC |
| Internal bar code: | GTTATCAACAGCAACGTGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 861 |
| LEAP-Seq percent confirming: | 99.1004 |
| LEAP-Seq n confirming: | 2644 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACCTGCAAGGACCTAGA |
| Suggested primer 2: | ATTGAGGCTAACAACCCACG |