| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.095989 |
| Chromosome: | chromosome 12 |
| Location: | 6213734 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGCCGCGTGAGCGCACCCAAACCCTAA |
| Internal bar code: | ATGGTGTTCTAAATGGCCACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 714 |
| LEAP-Seq percent confirming: | 99.6033 |
| LEAP-Seq n confirming: | 3515 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGACAAGATGGGTGTACT |
| Suggested primer 2: | TGCTCACTGCGTCAAATAGG |