| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.095992 |
| Chromosome: | chromosome 13 |
| Location: | 1840619 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575250 | (1 of 1) PTHR10663:SF114 - PROTEIN MON2 HOMOLOG | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCTTGGTTCTGGGAGTCTAGGTTAGCCA |
| Internal bar code: | GGTGGCCCCAGGGTGCGCGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 360 |
| LEAP-Seq percent confirming: | 99.6516 |
| LEAP-Seq n confirming: | 286 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGATCGCATTCCTACTCC |
| Suggested primer 2: | TGTACTGCTGTGGGTGTGGT |