| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.096021 |
| Chromosome: | chromosome_2 |
| Location: | 4997256 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g104201 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CATGCTGGCGCGAACCGCGGCTGCCACGTT |
| Internal bar code: | GTCGCCGCTGAATTCTCTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 432 |
| LEAP-Seq percent confirming: | 86.9729 |
| LEAP-Seq n confirming: | 1382 |
| LEAP-Seq n nonconfirming: | 207 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAAGTCTGGAGCATGCAA |
| Suggested primer 2: | CGGCTAGACTGTCGGTCTTC |