Insertion junction: LMJ.RY0402.096023_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.096023
Chromosome:chromosome 9
Location:2863201
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g388600 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTCGTTTGGTGTGGCTGCTGACCGAGTG
Internal bar code:CCGCAAGGGATCGGCGCACAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:121
LEAP-Seq percent confirming:47.9501
LEAP-Seq n confirming:538
LEAP-Seq n nonconfirming:584
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CAGATACGCTGCTCATTCCA
Suggested primer 2:ACACACACACACACGCACAC