| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.096024 |
| Chromosome: | chromosome 12 |
| Location: | 1368219 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g488350 | (1 of 2) K00464 - all-trans-8'-apo-beta-carotenal 15,15'-oxygenase (diox1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCGTGGATGAGAGCCACGTCCACTCCC |
| Internal bar code: | GAAACAAGTCGTATAAGCAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 46 |
| LEAP-Seq percent confirming: | 98.6254 |
| LEAP-Seq n confirming: | 287 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATACACGCAGCAGGGGAT |
| Suggested primer 2: | AGATGCATGACGCACAGAAG |