| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.096056 |
| Chromosome: | chromosome 14 |
| Location: | 925407 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613700 | (1 of 110) 3.4.19.12 - Ubiquitinyl hydrolase 1 / Ubiquitin thiolesterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCTTCTCGCCTTCTGAGATCCACCCCG |
| Internal bar code: | GGGACGGGGTCTAGCACCGTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 479 |
| LEAP-Seq percent confirming: | 82.4859 |
| LEAP-Seq n confirming: | 1022 |
| LEAP-Seq n nonconfirming: | 217 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTCGAGGACATGGTGAAC |
| Suggested primer 2: | CCCACCTCAAAGTCACCAGT |