Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.096071 |
Chromosome: | chromosome 16 |
Location: | 950898 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g648700 | (1 of 4) 3.6.3.29 - Molybdate-transporting ATPase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGTTTGCAAGTGGGGGTGTGTGTGGGGG |
Internal bar code: | GTGCCGCTCTGAGCAATCTCAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 35 |
LEAP-Seq percent confirming: | 87.5 |
LEAP-Seq n confirming: | 14 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACCACCTTAAACGCAACTGG |
Suggested primer 2: | AATTGTCTCGCGAGTGTGTG |