| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.096081 |
| Chromosome: | chromosome 3 |
| Location: | 3160276 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165200 | APG7 | Autophagy-related protein, ubiquitin-activating enzyme E1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACATGCCGACGTACGACCGGTAAGTTC |
| Internal bar code: | AGACTAAGGATGTCCAAGCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 61 |
| LEAP-Seq percent confirming: | 99.273 |
| LEAP-Seq n confirming: | 1502 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCAGCTGTGTGTGTGTG |
| Suggested primer 2: | CGATACCCACCATTTTACCG |