| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.096094 |
| Chromosome: | chromosome 6 |
| Location: | 3471317 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278096 | SELU2,SELENOU,SELU1 | Selenoprotein U2; (1 of 4) PF13911 - AhpC/TSA antioxidant enzyme (AhpC-TSA_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTCGTGTGTGGCGGCTCAAGTGCTGAA |
| Internal bar code: | TGGAACAGTCCCGAGGTACTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 696 |
| LEAP-Seq percent confirming: | 99.0698 |
| LEAP-Seq n confirming: | 213 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTTGAAGGACAGATTGC |
| Suggested primer 2: | TACGAGTACGGAGTACGGGG |